Mercapturic acid formation from sodium alkyl sulphates in the rat

Enzyme inhibition by sodium alkyl sulphates.

velocities in the forward and reverse directions was 54.5: 1 , somewhat lower than that previously reported (McQuate & Utter, 1959). Product-inhibition experiments with a non-saturating concentration of the constant substrate showed that in every case competitive inhibition occurs. This is consistent with a rapidequilibrium random Bi Bi mechanism for the reverse reaction, a similar mechanism to...

Initial intraorgan formation of mercapturic acid.

The disposition of S-benzyl-glutathione (BSG) in male Wistar rats was evaluated by the HPLC method to examine whether the kidney and liver contributed independently to the biosynthesis of S-benzyl-N-acetylcysteine (BNAc), a mercapturic acid (Chart 1). After intravenous injection, BSG was rapidly transported in both the kidney and the liver at a ratio of about 7:3. Simultaneously, a large amount...

The First Enzymatic Step in Mercapturic Acid Formation*

The purification of homogeneous glutathione S-transferases B and C from rat liver is described. Kinetic and physical properties of these enzymes are compared with those of homogeneous transferases A and E. The letter designations for the transferases are based on the reverse order of elution from carboxymethylcellulose, the purification step in which the transferases are separated from each oth...

The formation of mercapturic acids. 1. Formation of mercapturic acid and the levels of glutathione in tissues.

Formation of Mercapturic Acids

& Young (1959, 1960) showed that n-propylmercapturic acid was excreted by rats, rabbits, guinea pigs and mice to which 1-bromoor 1-iodopropane had been administered. Bray & James (1958) obtained evidence suggesting that 1-bromobutane, -pentane, -hexane, -heptane and -octane, and 1-nitro-ethane and -propane, formed mercapturic acids in the rabbit, and Barnes, James & Wood (1959) showed that a si...